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96 Gene Therapy and Ligament Healing 303 303 Table 3 Advantages and Disadvantages of Common Vectors for Gene Transfer Vector Viral Advantages Disadvantages Nonviral Hybrid HVJ liposomes Large-insert capacity High efficiency of transfection Infection of nondividing cells Transient gene expression Retrovirus Low toxicity Low immunogenicity High persistence of gene expression Low gene-insert capacity Infection only of dividing cells Oncogenesis Adenovirus Adeno-associated virus High efficiency daily forex news software transfection Infection of nondividing cells Toxicity Immunogenicity Herpes simplex virus Large insert capacity High efficiency of transfection Infection of nondividing cells Immunogenicity Liposomes DNAligand complexes Colloidal gold (gene gun) Low toxicity Low immunogenicity Easy preparation Low efficiency of transfection Low efficiency of transfection Transient gene expression Low toxicity Low immunogenicity High persistence of gene expression Infection of nondividing cells Low gene-insert capacity Acknowledgments I extend heartfelt thanks to my office staff, including Dr.
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